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Journal article

Super-resolution microscopy using standard fluorescent proteins in intact cells under cryo-conditions.

Abstract:

We introduce a super-resolution technique for fluorescence cryo-microscopy based on photoswitching of standard genetically encoded fluorescent marker proteins in intact mammalian cells at low temperature (81 K). Given the limit imposed by the lack of cryo-immersion objectives, current applications of fluorescence cryo-microscopy to biological specimens achieve resolutions between 400-500 nm only. We demonstrate that the single molecule characteristics of reversible photobleaching of mEGFP and...

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Publication status:
Published

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Publisher copy:
10.1021/nl501870p

Authors


Publisher:
American Chemical Society
Journal:
Nano letters More from this journal
Volume:
14
Issue:
7
Pages:
4171-4175
Publication date:
2014-07-01
DOI:
EISSN:
1530-6992
ISSN:
1530-6984
Language:
English
Keywords:
Pubs id:
pubs:469375
UUID:
uuid:89600c22-3dc0-4750-9197-880e58621d80
Local pid:
pubs:469375
Source identifiers:
469375
Deposit date:
2014-07-05

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