Super-resolution microscopy using standard fluorescent proteins in intact cells under cryo-conditions.

Journal article

We introduce a super-resolution technique for fluorescence cryo-microscopy based on photoswitching of standard genetically encoded fluorescent marker proteins in intact mammalian cells at low temperature (81 K). Given the limit imposed by the lack of cryo-immersion objectives, current applications of fluorescence cryo-microscopy to biological specimens achieve resolutions between 400-500 nm only. We demonstrate that the single molecule characteristics of reversible photobleaching of mEGFP and...

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Authors: Kaufmann, R; Schellenberger, P; Seiradake, E; Dobbie, I; Jones, E

• Publication status: Published
• Publisher: American Chemical Society
• Journal: Nano letters
• Volume: 14
• Issue: 7
• Pages: 4171-4175
• Publication date: 2014-07-01
• DOI: 10.1021/nl501870p
• EISSN: 1530-6992
• ISSN: 1530-6984
• Language: English
• Keywords: vitrification; Fluorescence cryo-microscopy; frozen-hydrated cells; single molecule blinking; low temperature; localization microscopy