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Construction of conditional analog-sensitive kinase alleles in the fission yeast Schizosaccharomyces pombe.

Abstract:

Reversible protein phosphorylation is a major regulatory mechanism in a cell. A chemical-genetic strategy to conditionally inactivate protein kinases has been developed recently. Mutating a single residue in the ATP-binding pocket confers sensitivity to small-molecule inhibitors. The inhibitor can only bind to the mutant kinase and not to any other wild-type kinase, allowing specific inactivation of the modified kinase. Here, we describe a protocol to construct conditional analog-sensitive ki...

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Publication status:
Published

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Publisher copy:
10.1038/nprot.2007.447

Authors


Journal:
Nature protocols More from this journal
Volume:
2
Issue:
11
Pages:
2996-3000
Publication date:
2007-01-01
DOI:
EISSN:
1750-2799
ISSN:
1754-2189
Language:
English
Keywords:
Pubs id:
pubs:98340
UUID:
uuid:893fd498-884a-4fa8-9f29-242fef3bc2df
Local pid:
pubs:98340
Source identifiers:
98340
Deposit date:
2013-11-17

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