Journal article
Rapid diagnostic tests as a source of dengue virus RNA for envelope gene amplification: a proof of concept
- Abstract:
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Molecular epidemiological data are key for dengue outbreak characterization and preparedness. However, sparse Dengue virus (DENV) molecular information is available in Laos because of limited resources. In this proof-of-concept study, we evaluated whether DENV1 RNA extracted from rapid diagnostic tests (RDTs) could be amplified and sequenced. The protocol for envelope gene amplification from RNA purified from RDTs was first assessed using viral isolate dilutions then conducted using 14 dengue...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
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(Preview, Version of record, pdf, 719.3KB, Terms of use)
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- Publisher copy:
- 10.4269/ajtmh.18-0831
Authors
Bibliographic Details
- Publisher:
- American Society of Tropical Medicine and Hygiene
- Journal:
- American Journal of Tropical Medicine and Hygiene More from this journal
- Volume:
- 101
- Issue:
- 2
- Pages:
- 451-455
- Publication date:
- 2019-06-24
- Acceptance date:
- 2019-05-06
- DOI:
- EISSN:
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1476-1645
- ISSN:
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0002-9637
- Pmid:
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31237231
Item Description
- Language:
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English
- Keywords:
- Pubs id:
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pubs:1024957
- UUID:
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uuid:88d70a17-0383-47b9-93a3-3fe37bf6a431
- Local pid:
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pubs:1024957
- Source identifiers:
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1024957
- Deposit date:
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2019-11-06
Terms of use
- Copyright holder:
- Cassidy-Seyoum et al
- Copyright date:
- 2019
- Notes:
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Copyright © 2019 by The American Society of Tropical Medicine and Hygiene. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted
use, distribution, and reproduction in any medium, provided the original author and source are credited.
- Licence:
- CC Attribution (CC BY)
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