Journal article icon

Journal article

Protein production and purification.

Abstract:
In selecting a method to produce a recombinant protein, a researcher is faced with a bewildering array of choices as to where to start. To facilitate decision-making, we describe a consensus 'what to try first' strategy based on our collective analysis of the expression and purification of over 10,000 different proteins. This review presents methods that could be applied at the outset of any project, a prioritized list of alternate strategies and a list of pitfalls that trip many new investigators.
Publication status:
Published

Actions


Access Document


Publisher copy:
10.1038/nmeth.f.202

Authors


Expand authors...
Journal:
Nature methods
Volume:
5
Issue:
2
Pages:
135-146
Publication date:
2008-02-05
DOI:
EISSN:
1548-7105
ISSN:
1548-7091
URN:
uuid:88c53547-b930-4a16-a155-4a9f77c2ab3b
Source identifiers:
29159
Local pid:
pubs:29159

Terms of use


Metrics


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP