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Hepatitis C virus glycoprotein E2 binding to CD81: the role of E1E2 cleavage and protein glycosylation in bioactivity.

Abstract:

The hepatitis C virus glycoproteins E1 and 2 have been expressed using recombinant baculoviruses following fusion to the carrier protein glutathione S-transferase (GST). Proteins were expressed singly and as an E1E2 polyprotein with and without an N-terminal affinity tag. Expression of the E1E2 polyprotein, even when preceded by GST, led to processing in insect cells and detection of an E1E2 complex that could be specifically purified by glutathione affinity chromatography. Baculovirus expres...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1006/viro.2000.0407

Authors


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Institution:
University of Oxford
Department:
Oxford, MSD, Biochemistry
Role:
Author
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Wellcome Trust More from this funder
Glaxo Wellcome Plc More from this funder
Publisher:
Elsevier Publisher's website
Journal:
Virology Journal website
Volume:
273
Issue:
1
Pages:
60-66
Publication date:
2000-01-01
DOI:
EISSN:
1096-0341
ISSN:
0042-6822
URN:
uuid:88b8023a-a6b7-4de3-b1e1-b54b48c4fd4e
Source identifiers:
99943
Local pid:
pubs:99943

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