Structures of CUG repeats in RNA. Potential implications for human genetic diseases.

Journal article

Triplet repeats that cause human genetic diseases have been shown to exhibit unusual compact structures in DNA, and in this paper we show that similar structures exist in shorter "normal length" CNG RNA. CUG and control RNAs were made chemically and by in vitro transcription. We find that "normal" short CUG RNAs migrate anomalously fast on non-denaturing gels, compared with control oligos of similar base composition. By contrast, longer tracts approaching clinically relevant lengths appear to...

Expand abstract

Authors: Pinheiro, P; Scarlett, G; Rodger, A; Rodger, P; Murray, A

• Publication status: Published
• Journal: Journal of biological chemistry
• Volume: 277
• Issue: 38
• Pages: 35183-35190
• Publication date: 2002-09-01
• DOI: 10.1074/jbc.m202235200
• EISSN: 1083-351X
• ISSN: 0021-9258
• Language: English
• Keywords: DNA Primers; Humans; Transcription, Genetic; Plasmids; Nucleic Acid Conformation; RNA; Genetic Diseases, Inborn; Base Sequence; Circular Dichroism; Models, Molecular; Trinucleotide Repeats