Isolation and characterization of antigen-specific plasmablasts using a novel flow cytometry-based Ig capture assay

Journal article

We report the development of a novel flow cytometry-based Ig capture assay (ICA) for the identification and sorting of individual Ab-secreting cells based on their Ag reactivity. The ICA represents a fast and versatile tool for single-cell sorting of peripheral plasmablasts, streamlining subsequent Ab analysis, and cloning. We demonstrate the utility of the assay by isolating Ag-reactive plasmablasts from cryopreserved PBMC obtained from volunteers vaccinated with a recombinant HIV envelope p...

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Authors: Pinder, C; Kratochvil, S; Cizmeci, D; Muir, L; Guo, Y

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: American Association of Immunologists
• Journal: Journal of Immunology
• Volume: 199
• Issue: 12
• Pages: 4180-4188
• Publication date: 2017-12-04
• Acceptance date: 2017-10-10
• DOI: 10.4049/jimmunol.1701253
• EISSN: 1550-6606
• ISSN: 0022-1767
• Pmid: 29118244
• Language: English
• Keywords: Humans; Follow-Up Studies; Vaccination; Tetanus Toxoid; Cell Separation; Hepatitis B Surface Antigens; HIV Antibodies; Tetanus Toxin; Peptide Fragments; Antibodies, Bacterial; env Gene Products, Human Immunodeficiency Virus; Cryopreservation; Flow Cytometry; HEK293 Cells; Hepatitis B Vaccines; Enzyme-Linked Immunospot Assay; Antigens, Bacterial; Blood Preservation; Plasma Cells; Antigen-Antibody Reactions; AIDS Vaccines