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DNA adopts normal B-form upon incorporation of highly fluorescent DNA base analogue tC: NMR structure and UV-Vis spectroscopy characterization.

Abstract:

The influence of the highly fluorescent tricyclic cytosine base analogue (tC) on duplex DNA conformation is investigated. The duplex properties are characterized by absorbance and circular dichroism (CD) for all combinations of neighbouring bases to tC, and an NMR structure is determined for one tC-containing sequence. For the oligonucleotides with one tC incorporated instead of cytosine, the melting temperature is increased on average by 2.7 degrees C above that for the unmodified ones. CD s...

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Publication status:
Published

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Publisher copy:
10.1093/nar/gkh844

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Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Organic Chemistry
Role:
Author
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Journal:
Nucleic acids research
Volume:
32
Issue:
17
Pages:
5087-5095
Publication date:
2004-01-01
DOI:
EISSN:
1362-4962
ISSN:
0305-1048
Source identifiers:
400211
Language:
English
Keywords:
Pubs id:
pubs:400211
UUID:
uuid:8813495a-4138-4087-bffa-584b8d40f40a
Local pid:
pubs:400211
Deposit date:
2013-11-16

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