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Factors affecting the spontaneous cell-mediated cytotoxicity of intestinal mononuclear cells.

Abstract:

This study was performed to determine what factors related to the enzymatic isolation technique and assay conditions may influence the measurement of spontaneous cell-mediated cytotoxicity (SCMC) of mononuclear cells (MNC) isolated from human intestinal mucosa. In 18 studies, the SCMC of freshly isolated cells was 1.8 +/- 0.4% but increased to 12 +/- 3% following 24 hr culture without a change in the proportion of cells with the NK phenotype (Leu-7+). The SCMC tended to plateau with more prol...

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Publication status:
Published

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Authors


Gibson, PR More by this author
Hermanowicz, A More by this author
Jewell, DP More by this author
Journal:
Immunology
Volume:
53
Issue:
2
Pages:
267-274
Publication date:
1984-10-05
EISSN:
1365-2567
ISSN:
0019-2805
URN:
uuid:880f27e3-1e3c-4b2b-af25-2ff63f5307d4
Source identifiers:
135891
Local pid:
pubs:135891

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