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The circularly permuted yellow fluorescent protein cpYFP that has been used as a superoxide probe is highly responsive to pH but not superoxide in mitochondria: implications for the existence of superoxide 'flashes'.

Abstract:
The properties of a cpYFP [circularly permuted YFP (yellow fluorescent protein)] reported to act as a superoxide sensor have been re-examined in Arabidopsis mitochondria. We have found that the probe has high pH sensitivity and that dynamics in the cpYFP signal disappeared when the matrix pH was clamped by nigericin. In contrast, genetic and pharmacological manipulation of matrix superoxide had no detectable effect on the cpYFP signal. These findings question the existence of superoxide flashes in mitochondria.
Publication status:
Published

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Publisher copy:
10.1042/bj20110883

Authors


More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Plant Sciences
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Plant Sciences
Role:
Author
Journal:
Biochemical journal
Volume:
437
Issue:
3
Pages:
381-387
Publication date:
2011-08-01
DOI:
EISSN:
1470-8728
ISSN:
0264-6021
Source identifiers:
166451
Language:
English
Keywords:
Pubs id:
pubs:166451
UUID:
uuid:874d54ab-59d1-4352-aa7f-06a015a36371
Local pid:
pubs:166451
Deposit date:
2012-12-19

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