Journal article
A streamlined method for systematic, high resolution in situ analysis of mRNA distribution in plants.
- Abstract:
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BACKGROUND: In situ hybridisation can provide cellular, and in some cases sub-cellular, resolution of mRNA levels within multicellular organisms and is widely used to provide spatial and temporal information on gene expression. However, standard protocols are complex and laborious to implement, restricting analysis to one or a few genes at any one time. Whole-mount and reverse transcriptase-PCR (RT-PCR) based protocols increase throughput, but can compromise both specificity and resolution. ...
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- Publisher copy:
- 10.1186/1746-4811-1-8
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Authors
Bibliographic Details
- Journal:
- Plant methods
- Volume:
- 1
- Issue:
- 1
- Pages:
- 8
- Publication date:
- 2005-01-01
- DOI:
- EISSN:
-
1746-4811
- Source identifiers:
-
50113
Item Description
- Language:
- English
- Pubs id:
-
pubs:50113
- UUID:
-
uuid:871fa02b-b425-4e3f-b43f-59cd9c648ec5
- Local pid:
- pubs:50113
- Deposit date:
- 2012-12-19
Terms of use
- Copyright date:
- 2005
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