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A streamlined method for systematic, high resolution in situ analysis of mRNA distribution in plants.

Abstract:

BACKGROUND: In situ hybridisation can provide cellular, and in some cases sub-cellular, resolution of mRNA levels within multicellular organisms and is widely used to provide spatial and temporal information on gene expression. However, standard protocols are complex and laborious to implement, restricting analysis to one or a few genes at any one time. Whole-mount and reverse transcriptase-PCR (RT-PCR) based protocols increase throughput, but can compromise both specificity and resolution. ...

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Publisher copy:
10.1186/1746-4811-1-8

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Institution:
University of Oxford
Department:
Oxford, MPLS, Plant Sciences
Role:
Author
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Journal:
Plant methods
Volume:
1
Issue:
1
Pages:
8
Publication date:
2005-01-01
DOI:
EISSN:
1746-4811
URN:
uuid:871fa02b-b425-4e3f-b43f-59cd9c648ec5
Source identifiers:
50113
Local pid:
pubs:50113
Language:
English

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