- Abstract:
-
BACKGROUND: In situ hybridisation can provide cellular, and in some cases sub-cellular, resolution of mRNA levels within multicellular organisms and is widely used to provide spatial and temporal information on gene expression. However, standard protocols are complex and laborious to implement, restricting analysis to one or a few genes at any one time. Whole-mount and reverse transcriptase-PCR (RT-PCR) based protocols increase throughput, but can compromise both specificity and resolution. ...
Expand abstract - Publisher copy:
- 10.1186/1746-4811-1-8
-
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- Journal:
- Plant methods
- Volume:
- 1
- Issue:
- 1
- Pages:
- 8
- Publication date:
- 2005-01-01
- DOI:
- EISSN:
-
1746-4811
- URN:
-
uuid:871fa02b-b425-4e3f-b43f-59cd9c648ec5
- Source identifiers:
-
50113
- Local pid:
- pubs:50113
- Language:
- English
- Copyright date:
- 2005
Journal article
A streamlined method for systematic, high resolution in situ analysis of mRNA distribution in plants.
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