Journal article icon

Journal article

A streamlined method for systematic, high resolution in situ analysis of mRNA distribution in plants.

Abstract:

BACKGROUND: In situ hybridisation can provide cellular, and in some cases sub-cellular, resolution of mRNA levels within multicellular organisms and is widely used to provide spatial and temporal information on gene expression. However, standard protocols are complex and laborious to implement, restricting analysis to one or a few genes at any one time. Whole-mount and reverse transcriptase-PCR (RT-PCR) based protocols increase throughput, but can compromise both specificity and resolution. ...

Expand abstract

Actions


Access Document


Publisher copy:
10.1186/1746-4811-1-8

Authors


More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Plant Sciences
Role:
Author
Expand authors...
Journal:
Plant methods
Volume:
1
Issue:
1
Pages:
8
Publication date:
2005-01-01
DOI:
EISSN:
1746-4811
Source identifiers:
50113
Language:
English
Pubs id:
pubs:50113
UUID:
uuid:871fa02b-b425-4e3f-b43f-59cd9c648ec5
Local pid:
pubs:50113
Deposit date:
2012-12-19

Terms of use


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP