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Journal article

Antibody for detecting p53 protein by immunohistochemistry in normal tissues.

Abstract:

AIMS: To establish whether PAb248 recognises human p53 as well as murine p53 and if so, to determine its distribution in normal tissues. METHODS: The ability of PAb248 to recognise human p53 was established by analysis of the human osteosarcoma derived Saos-2 cell line, which lacks the p53 gene, before and after transfection with p53 cDNA, using western blotting and immunoprecipitation. Immunostaining on normal tissues and cell lines was carried out using an immunoperoxidase technique. The tw...

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Publication status:
Published

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Publisher copy:
10.1136/jcp.47.7.592

Authors


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Institution:
University of Oxford
Department:
Oxford, MSD, RDM, Clinical Lab Sciences
Micklem, K More by this author
Pulford, K More by this author
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Publisher:
BMJ Publishing Group
Journal:
Journal of clinical pathology
Volume:
47
Issue:
7
Pages:
592-596
Publication date:
1994-07-05
DOI:
EISSN:
1472-4146
ISSN:
0021-9746
URN:
uuid:86e1f90a-7c29-4322-83c8-8fc752ef78db
Source identifiers:
486729
Local pid:
pubs:486729

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