- Abstract:
-
RNA polymerase II transcribes most eukaryotic genes. Its catalytic subunit was tagged with green fluorescent protein and expressed in Chinese hamster cells bearing a mutation in the same subunit; it complemented the defect and so was functional. Photobleaching revealed two kinetic fractions of polymerase in living nuclei: approximately 75% moved rapidly, but approximately 25% was transiently immobile (association t1/2 approximately 20 min) and transcriptionally active, as incubation with 5,6-...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1083/jcb.200206019
-
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- Journal:
- The Journal of cell biology
- Volume:
- 159
- Issue:
- 5
- Pages:
- 777-782
- Publication date:
- 2002-12-05
- DOI:
- EISSN:
-
1540-8140
- ISSN:
-
0021-9525
- URN:
-
uuid:8693d103-aa3e-4bc8-adc1-24d30676ac67
- Source identifiers:
-
19298
- Local pid:
- pubs:19298
- Language:
- English
- Keywords:
- Copyright date:
- 2002
Journal article
The transcription cycle of RNA polymerase II in living cells.
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