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The transcription cycle of RNA polymerase II in living cells.

Abstract:

RNA polymerase II transcribes most eukaryotic genes. Its catalytic subunit was tagged with green fluorescent protein and expressed in Chinese hamster cells bearing a mutation in the same subunit; it complemented the defect and so was functional. Photobleaching revealed two kinetic fractions of polymerase in living nuclei: approximately 75% moved rapidly, but approximately 25% was transiently immobile (association t1/2 approximately 20 min) and transcriptionally active, as incubation with 5,6-...

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Publication status:
Published

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Publisher copy:
10.1083/jcb.200206019

Authors


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Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
Role:
Author
Journal:
The Journal of cell biology
Volume:
159
Issue:
5
Pages:
777-782
Publication date:
2002-12-05
DOI:
EISSN:
1540-8140
ISSN:
0021-9525
URN:
uuid:8693d103-aa3e-4bc8-adc1-24d30676ac67
Source identifiers:
19298
Local pid:
pubs:19298

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