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The transcription cycle of RNA polymerase II in living cells.

Abstract:

RNA polymerase II transcribes most eukaryotic genes. Its catalytic subunit was tagged with green fluorescent protein and expressed in Chinese hamster cells bearing a mutation in the same subunit; it complemented the defect and so was functional. Photobleaching revealed two kinetic fractions of polymerase in living nuclei: approximately 75% moved rapidly, but approximately 25% was transiently immobile (association t1/2 approximately 20 min) and transcriptionally active, as incubation with 5,6-...

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Publication status:
Published

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Publisher copy:
10.1083/jcb.200206019

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Institution:
University of Oxford
Division:
MSD
Department:
Pathology Dunn School
Role:
Author
Journal:
Journal of cell biology
Volume:
159
Issue:
5
Pages:
777-782
Publication date:
2002-12-01
DOI:
EISSN:
1540-8140
ISSN:
0021-9525
Source identifiers:
19298

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