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Journal article

Selenomethionine labeling of large biological macromolecular complexes: probing the structure of marine bacterial virus PM2.

Abstract:

There is a need for improved tools for labeling protein species within large macromolecular assemblies. Here we describe a method for the efficient selenomethionine labeling of the membrane-containing bacterial virus PM2 for structural studies. By examining potential host cells a strain was found which was auxotrophic for methionine, and by performing a multiparameter search of conditions it was possible to derive a robust protocol which simultaneously minimized the toxic effects of the selen...

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Publication status:
Published

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Publisher copy:
10.1016/j.jsb.2007.10.013

Authors


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Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Medicine, Structural Biology
Role:
Author
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Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Medicine, Structural Biology
Role:
Author
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Journal:
Journal of structural biology
Volume:
161
Issue:
2
Pages:
204-210
Publication date:
2008-02-05
DOI:
EISSN:
1095-8657
ISSN:
1047-8477
URN:
uuid:851743d3-1b7e-4fa9-a41f-c2f38671fa03
Source identifiers:
9739
Local pid:
pubs:9739

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