There is a need for improved tools for labeling protein species within large macromolecular assemblies. Here we describe a method for the efficient selenomethionine labeling of the membrane-containing bacterial virus PM2 for structural studies. By examining potential host cells a strain was found which was auxotrophic for methionine, and by performing a multiparameter search of conditions it was possible to derive a robust protocol which simultaneously minimized the toxic effects of the selen...Expand abstract
- Publication status:
- Publisher copy:
- Copyright date:
Selenomethionine labeling of large biological macromolecular complexes: probing the structure of marine bacterial virus PM2.
Views and Downloads
If you are the owner of this record, you can report an update to it here: Report update to this record