Role of Fyn in the rearrangement of tubulin cytoskeleton induced through TCR.

Journal article

The translocation of the microtubule-organizing center (MTOC), its associated signaling complex, and the secretory apparatus is the most characteristic early event that involves the tubulin cytoskeleton of T or NK cells after their interaction with APC or target cells. Our results show that Fyn kinase activity is essential for MTOC reorientation in an Ag-dependent system. Moreover, T cells from Fyn-deficient mice are unable to rearrange their tubulin cytoskeleton in response to anti-CD3-coated beads. Analysis of conjugates of T cells from transgenic OT-I mice with dendritic cells revealed that an antagonist peptide induces translocation of the MTOC, and that this process is impaired in T cells from Fyn(-/-) OT-I mice. In addition, Fyn deficiency significantly affects the MTOC relocation mediated by agonist peptide stimulation. These results reveal Fyn to be a key regulator of tubulin cytoskeleton reorganization in T cells.

Authors: Martín-Cófreces, N; Sancho, D; Fernández, E; Vicente-Manzanares, M; Gordón-Alonso, M

• Publication status: Published
• Journal: Journal of Immunology
• Volume: 176
• Issue: 7
• Pages: 4201-4207
• Publication date: 2006-04-01
• DOI: 10.4049/jimmunol.176.7.4201
• EISSN: 1550-6606
• ISSN: 0022-1767
• Language: English
• Keywords: Tubulin; Cell Line; Receptors, Antigen, T-Cell; Mice; Peptide Fragments; Mice, Knockout; Animals; Enzyme Activation; Proto-Oncogene Proteins c-fyn; Cytoskeleton; Adaptor Proteins, Signal Transducing; T-Lymphocytes; Humans