Active RNA polymerase I is fixed within the nucleus of HeLa cells.

Journal article

We have investigated whether active RNA polymerase I, the enzyme responsible for transcribing ribosomal RNA, is immobilized by attachment to a large subnuclear structure in HeLa cells. As unphysiological salt concentrations induce artifacts, we have used isotonic conditions throughout the preparative and analytic procedures. Cells are encapsulated in agarose microbeads and lysed in Triton and a 'physiological' buffer; then soluble proteins and RNA diffuse out through the agarose pores to leav...

Expand abstract

Authors: Dickinson, P; Cook, P; Jackson, D

• Publication status: Published
• Journal: EMBO journal
• Volume: 9
• Issue: 7
• Pages: 2207-2214
• Publication date: 1990-07-01
• EISSN: 1460-2075
• ISSN: 0261-4189
• Language: English
• Keywords: Cell Nucleus; Chromatin; Transcription, Genetic; RNA Polymerase I; Humans; Globins; Hela Cells; Dactinomycin; DNA, Ribosomal; Restriction Mapping; Models, Structural; Nucleic Acid Hybridization; Kinetics; Nucleic Acid Conformation