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From clinical sample to complete genome: Comparing methods for the extraction of HIV-1 RNA for high-throughput deep sequencing

Abstract:

The BEEHIVE (Bridging the Evolution and Epidemiology of HIV in Europe) project aims to analyse nearly-complete viral genomes from >3000 HIV-1 infected Europeans using high-throughput deep sequencing techniques to investigate the virus genetic contribution to virulence. Following the development of a computational pipeline, including a new de novo assembler for RNA virus genomes, to generate larger contiguous sequences (contigs) from the abundance of short sequence reads that characterise t...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1016/j.virusres.2016.08.004

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Publisher:
Elsevier Publisher's website
Journal:
Virus Research Journal website
Volume:
239
Pages:
10-16
Publication date:
2016-08-04
DOI:
EISSN:
1872-7492
ISSN:
0168-1702
Pubs id:
pubs:675656
URN:
uri:84962239-f2a1-4959-91c3-a618b6837976
UUID:
uuid:84962239-f2a1-4959-91c3-a618b6837976
Local pid:
pubs:675656

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