Ceramide glycosylation and fatty acid hydroxylation influence serological reactivity in Trypanosoma cruzi glycosphingolipids.

Journal article

Ceramide mono (CMH) or dihexoside (CDH) fractions from Trypanosoma cruzi (Dm28c clone) were identified as glucosyl and lactosylceramides containing non-hydroxylated fatty acids. The di-glycosylated form was much more efficiently recognized by sera from T. cruzi-immunized rabbits, indicating that glycosylation influences antigenicity. Fatty acid hydroxylation was also a determinant of serological reactivity, since an alpha-hydroxylated CMH, only present at the Y clone, was recognized by the hy...

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Authors: Villas-Boas, M; Wait, R; Silva, R; Rodrigues, M; Barreto-Bergter, E

• Publication status: Published
• Journal: FEMS microbiology letters
• Volume: 244
• Issue: 1
• Pages: 47-52
• Publication date: 2005-03-01
• DOI: 10.1016/j.femsle.2005.01.024
• EISSN: 1574-6968
• ISSN: 0378-1097
• Language: English
• Keywords: Glycosphingolipids; Fatty Acids; Rabbits; Spectrometry, Mass, Fast Atom Bombardment; Male; Glycosylation; Animals; Trypanosoma cruzi; Hydroxylation; Antibodies, Protozoan; Antigens, Protozoan; Ceramides