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Journal article

Genome-wide discovery of human splicing branchpoints.

Abstract:

During the splicing reaction, the 5' intron end is joined to the branchpoint nucleotide, selecting the next exon to incorporate into the mature RNA and forming an intron lariat, which is excised. Despite a critical role in gene splicing, the locations and features of human splicing branchpoints are largely unknown. We use exoribonuclease digestion and targeted RNA-sequencing to enrich for sequences that traverse the lariat junction and, by split and inverted alignment, reveal the branchpoint....

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Publication status:
Published

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Publisher copy:
10.1101/gr.182899.114

Authors


Mercer, TR More by this author
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Institution:
University of Oxford
Department:
Oxford, MSD, Physiology Anatomy and Genetics
Andersen, SB More by this author
Brunck, ME More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Physiology Anatomy and Genetics
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Journal:
Genome research
Volume:
25
Issue:
2
Pages:
290-303
Publication date:
2015-02-05
DOI:
EISSN:
1549-5469
ISSN:
1088-9051
URN:
uuid:83f44876-5889-4540-b3ed-5c66b7fcd105
Source identifiers:
502481
Local pid:
pubs:502481
Language:
English

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