A new method for RAPD primers selection based on primer bias in nucleotide sequence data.

Journal article

Sequence analysis has proved that decamer nucleotides, used as primers of RAPD (random amplified polymorphic DNA), differ with each other greatly in number of annealing sites in the Arabidopsis thaliana genome. It is called the 'primer bias' by the authors. The biased primers produce a highly variable number of amplicons by polymerase chain reaction (PCR). The number of amplicons is proved to correlate with the number of annealing sites. Therefore, a statistical method is proposed for selecti...

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Authors: Li, J; Pei, G; Pang, H; Bilderbeck, A; Chen, S

• Publication status: Published
• Journal: Journal of biotechnology
• Volume: 126
• Issue: 4
• Pages: 415-423
• Publication date: 2006-12-01
• DOI: 10.1016/j.jbiotec.2006.05.003
• EISSN: 1873-4863
• ISSN: 0168-1656
• Language: English
• Keywords: Genome, Plant; Polymerase Chain Reaction; DNA Primers; Expressed Sequence Tags; Arabidopsis; Base Sequence; Random Amplified Polymorphic DNA Technique