Journal article
Four-dimensional imaging of living chondrocytes in cartilage using confocal microscopy: a pragmatic approach.
- Abstract:
-
Regulation of cell volume is a fundamental cellular homeostatic mechanism in the face of osmotic stress. In normal articular cartilage, chondrocytes are exposed to a changing osmotic environment. We present a comprehensive protocol for studying the volume regulatory behavior of chondrocytes within intact cartilage tissue using confocal laser-scanning microscopy. Our data acquisition regime optimizes both signal-to-noise and cell viability during time-lapsed three-dimensional (3-D) (x, y, z, t...
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- Publication status:
- Published
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Bibliographic Details
- Journal:
- American journal of physiology
- Volume:
- 272
- Issue:
- 3 Pt 1
- Pages:
- C1040-C1051
- Publication date:
- 1997-03-01
- ISSN:
-
0002-9513
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:45437
- UUID:
-
uuid:83af14a7-6c0b-40ad-9dc3-d5e6f3a8deda
- Local pid:
- pubs:45437
- Source identifiers:
-
45437
- Deposit date:
- 2012-12-19
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- Copyright date:
- 1997
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