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Bioprocess applications of a Sindbis virus-based temperature-inducible expression system.

Abstract:

The production and study of toxic proteins requires inducible expression systems with low basal level expression and high inducibility. Here, we describe bioprocess applications of the pCytTS temperature-regulatable Sindbis virus replicon-based expression system. We used green fluorescent protein as a marker protein to optimize the selection of stable transfected clones with increased expression levels. Using the optimized protocol, clones were constructed that produced the growth-inhibiting,...

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Publication status:
Published

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Publisher copy:
10.1002/bit.10311

Authors


Boorsma, M More by this author
Marrero, A More by this author
Fischer, R More by this author
Bailey, JE More by this author
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Journal:
Biotechnology and bioengineering
Volume:
79
Issue:
6
Pages:
602-609
Publication date:
2002-09-05
DOI:
EISSN:
1097-0290
ISSN:
0006-3592
URN:
uuid:832bc210-4eb6-43df-8d80-8e37b874c328
Source identifiers:
469703
Local pid:
pubs:469703

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