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Reverse-phase versus sandwich antibody microarray, technical comparison from a clinical perspective.

Abstract:

Protein microarrays are powerful tools to quantify and characterize proteins in multiplex assays. They have great potential within clinical diagnostics and prognostics, as they minimize consumption of both analyte and biological sample. Assays that do not require labeling of the biological specimen, henceforth called label-free, are vital for ease of clinical sample processing. Here, we evaluate two label-free techniques, reverse-phase and sandwich antibody assays, using microarrays on high-p...

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Publisher copy:
10.1021/ac0709955

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Journal:
Analytical chemistry
Volume:
79
Issue:
15
Pages:
5817-5825
Publication date:
2007-08-05
DOI:
EISSN:
1520-6882
ISSN:
0003-2700
URN:
uuid:81e1aa5b-c205-4edd-ba03-7ef355557a2c
Source identifiers:
365942
Local pid:
pubs:365942

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