Adaption of neutral protease activity for islet isolation from the long-term two-layer method-stored pig pancreas.

Journal article

BACKGROUND: Islet release from the pancreas is mediated by both collagenase and neutral protease (NP), a critical effector of islet integrity. To prove the hypothesis that adjustment of NP reduces islet damage after prolonged ischemia, adult pig pancreata were digested after 7-hour preservation by the two-layer method (TLM) using a 2-component enzyme blend consisting of collagenase NB-8 and NP. METHODS: After intraductal University of Wisconsin (UW) flush resected pancreata were distended wit...

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Authors: Brandhorst, D; Iken, M; Brendel, MD; Bretzel, R; Brandhorst, H

• Journal: Transplantation proceedings
• Volume: 37
• Issue: 1
• Pages: 458-459
• Publication date: 2005-01-01
• DOI: 10.1016/j.transproceed.2004.12.035
• EISSN: 1873-2623
• ISSN: 0041-1345
• Language: English
• Keywords: Raffinose; Organ Preservation Solutions; Insulin; Collagenases; Organ Preservation; Allopurinol; Endopeptidases; Swine; Glutathione; Animals; Pancreas; Islets of Langerhans; Adenosine