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Quantitative regulation of intracellular endothelial nitric-oxide synthase (eNOS) coupling by both tetrahydrobiopterin-eNOS stoichiometry and biopterin redox status: insights from cells with tet-regulated GTP cyclohydrolase I expression.

Abstract:

Tetrahydrobiopterin (BH4) is a critical determinant of endothelial nitric-oxide synthase (eNOS) activity. In the absence of BH4, eNOS becomes "uncoupled" and generates superoxide rather than NO. However, the stoichiometry of intracellular BH4/eNOS interactions is not well defined, and it is unclear whether intracellular BH4 deficiency alone is sufficient to induce eNOS uncoupling. To address these questions, we developed novel cell lines with tet-regulated expression of human GTP cyclohydrola...

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Publication status:
Published

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Publisher copy:
10.1074/jbc.m805403200

Authors


Crabtree, MJ More by this author
Tatham, AL More by this author
Al-Wakeel, Y More by this author
Warrick, N More by this author
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Journal:
The Journal of biological chemistry
Volume:
284
Issue:
2
Pages:
1136-1144
Publication date:
2009-01-05
DOI:
EISSN:
1083-351X
ISSN:
0021-9258
URN:
uuid:809f7dcd-b0b6-48fe-aa1d-1f3687b1bfab
Source identifiers:
104902
Local pid:
pubs:104902

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