Journal article
Deep RNA-seq of male and female murine sensory neuron subtypes after nerve injury
- Abstract:
- Dorsal root ganglia (DRG) neurons have been well described for their role in driving both acute and chronic pain. Although nerve injury is known to cause transcriptional dysregulation, how this differs across neuronal subtypes and the impact of sex is unclear. Here, we study the deep transcriptional profiles of multiple murine DRG populations in early and late pain states while considering sex. We have exploited currently available transgenics to label numerous subpopulations for fluorescent-activated cell sorting and subsequent transcriptomic analysis. Using bulk tissue samples, we are able to circumvent the issues of low transcript coverage and drop-outs seen with single-cell data sets. This increases our power to detect novel and even subtle changes in gene expression within neuronal subtypes and discuss sexual dimorphism at the neuronal subtype level. We have curated this resource into an accessible database for other researchers (https://livedataoxford.shinyapps.io/drg-directory/). We see both stereotyped and unique subtype signatures in injured states after nerve injury at both an early and late timepoint. Although all populations contribute to a general injury signature, subtype enrichment changes can also be seen. Within populations, there is not a strong intersection of sex and injury, but previously unknown sex differences in naïve states—particularly in Aβ-RA + Aδ-low threshold mechanoreceptors—still contribute to differences in injured neurons.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
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(Preview, Version of record, pdf, 2.8MB, Terms of use)
-
- Publisher copy:
- 10.1097/j.pain.0000000000002934
Authors
- Publisher:
- Wolters Kluwer
- Journal:
- Pain More from this journal
- Volume:
- 164
- Issue:
- 10
- Pages:
- 2196-2215
- Place of publication:
- United States
- Publication date:
- 2023-06-06
- Acceptance date:
- 2023-02-05
- DOI:
- EISSN:
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1872-6623
- ISSN:
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0304-3959
- Pmid:
-
37318015
- Language:
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English
- Keywords:
- Pubs id:
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1439157
- Local pid:
-
pubs:1439157
- Deposit date:
-
2023-12-19
Terms of use
- Copyright holder:
- Barry et al
- Copyright date:
- 2023
- Rights statement:
- © 2023 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the International Association for the Study of Pain. This is an open access article distributed under the Creative Commons Attribution License 4.0 (CCBY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
- Licence:
- CC Attribution (CC BY)
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