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Robust phosphoproteome enrichment using monodisperse microsphere-based immobilized titanium (IV) ion affinity chromatography.

Abstract:

Mass spectrometry (MS)-based proteomics has become the preferred tool for the analysis of protein phosphorylation. To be successful at such an endeavor, there is a requirement for an efficient enrichment of phosphopeptides. This is necessary because of the substoichiometric nature of phosphorylation at a given site and the complexity of the cell. Recently, new alternative materials have emerged that allow excellent and robust enrichment of phosphopeptides. These monodisperse microsphere-based...

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Publication status:
Published

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Publisher copy:
10.1038/nprot.2013.010

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Journal:
Nature protocols
Volume:
8
Issue:
3
Pages:
461-480
Publication date:
2013-03-05
DOI:
EISSN:
1750-2799
ISSN:
1754-2189
URN:
uuid:7f969e34-1061-4315-a171-259be64e4f6c
Source identifiers:
384542
Local pid:
pubs:384542

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