Journal article
Robust phosphoproteome enrichment using monodisperse microsphere-based immobilized titanium (IV) ion affinity chromatography.
- Abstract:
-
Mass spectrometry (MS)-based proteomics has become the preferred tool for the analysis of protein phosphorylation. To be successful at such an endeavor, there is a requirement for an efficient enrichment of phosphopeptides. This is necessary because of the substoichiometric nature of phosphorylation at a given site and the complexity of the cell. Recently, new alternative materials have emerged that allow excellent and robust enrichment of phosphopeptides. These monodisperse microsphere-based...
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- Publication status:
- Published
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- Publisher copy:
- 10.1038/nprot.2013.010
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Authors
Bibliographic Details
- Journal:
- Nature protocols
- Volume:
- 8
- Issue:
- 3
- Pages:
- 461-480
- Publication date:
- 2013-03-01
- DOI:
- EISSN:
-
1750-2799
- ISSN:
-
1754-2189
- Source identifiers:
-
384542
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:384542
- UUID:
-
uuid:7f969e34-1061-4315-a171-259be64e4f6c
- Local pid:
- pubs:384542
- Deposit date:
- 2013-11-16
Terms of use
- Copyright date:
- 2013
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