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MULTIPRINS: a method for multicolour primed in situ labelling.

Abstract:
With this fast and simple MULTIPRINS protocol we show simultaneous mapping of multiple repetitive sequences by sequential cycles of primed in situ (PRINS) labelling with various primers and reporter molecules. Differentially labelled DNA sequences synthesized in situ were visualized by directly incorporated rhodamine-4-dUTP, by fluorescein isothiocyanate and Cy5 using digital imaging microscopy. This further development of the PRINS method enhances its potential as an alternative to traditional in situ hybridization.

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Publisher copy:
10.1007/bf00710131

Authors


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Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Medicine, WTC Human Genetics
Role:
Author
Journal:
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology
Volume:
1
Issue:
4
Pages:
257-260
Publication date:
1993-11-05
DOI:
EISSN:
1573-6849
ISSN:
0967-3849
URN:
uuid:7f8c7942-e3be-4f51-b10f-094286032a6c
Source identifiers:
35797
Local pid:
pubs:35797

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