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Mechanism and rates of exchange of L7/L12 between ribosomes and the effects of binding EF-G.

Abstract:

The ribosomal stalk complex binds and recruits translation factors to the ribosome during protein biosynthesis. In Escherichia coli the stalk is composed of protein L10 and four copies of L7/L12. Despite the crucial role of the stalk, mechanistic details of L7/L12 subunit exchange are not established. By incubating isotopically labeled intact ribosomes with their unlabeled counterparts we monitored the exchange of the labile stalk proteins by recording mass spectra as a function of time. On t...

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Publication status:
Published

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Publisher copy:
10.1021/cb300081s

Authors


Marcoux, J More by this author
Gordiyenko, Y More by this author
Koripella, RK More by this author
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Journal:
ACS chemical biology
Volume:
7
Issue:
6
Pages:
1120-1127
Publication date:
2012-06-05
DOI:
EISSN:
1554-8937
ISSN:
1554-8929
URN:
uuid:7f72f556-4a2e-41ec-ac5f-a5930fa17139
Source identifiers:
325164
Local pid:
pubs:325164

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