Mechanism and rates of exchange of L7/L12 between ribosomes and the effects of binding EF-G.

Journal article

The ribosomal stalk complex binds and recruits translation factors to the ribosome during protein biosynthesis. In Escherichia coli the stalk is composed of protein L10 and four copies of L7/L12. Despite the crucial role of the stalk, mechanistic details of L7/L12 subunit exchange are not established. By incubating isotopically labeled intact ribosomes with their unlabeled counterparts we monitored the exchange of the labile stalk proteins by recording mass spectra as a function of time. On t...

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Authors: Deroo, S; Hyung, S; Marcoux, J; Gordiyenko, Y; Koripella, R

• Publication status: Published
• Journal: ACS chemical biology
• Volume: 7
• Issue: 6
• Pages: 1120-1127
• Publication date: 2012-06-01
• DOI: 10.1021/cb300081s
• EISSN: 1554-8937
• ISSN: 1554-8929
• Language: English
• Keywords: Ribosomal Proteins; Protein Binding; Escherichia coli; Protein Subunits; Protein Multimerization; Kinetics; Mass Spectrometry; Escherichia coli Proteins; Ribosomes; Peptide Elongation Factor G