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Quasi-simultaneous multiplane calcium imaging of neuronal circuits

Abstract:

Two-photon excitation fluorescence microscopy is widely used to study the activity of neuronal circuits. However, the fast imaging is typically constrained to a single lateral plane for a standard microscope design. Given that cortical neuronal networks in a mouse brain are complex three-dimensional structures organised in six histologically defined layers which extend over many hundreds of micrometres, there is a strong demand for microscope systems that can record neuronal signalling in vol...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1364/boe.10.000267

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Institution:
University of Oxford
Division:
MPLS Division
Department:
Engineering Science
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Physiology Anatomy and Genetics
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Physiology Anatomy and Genetics
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Physiology Anatomy and Genetics
Oxford college:
Worcester College
ORCID:
0000-0002-2566-5426
More by this author
Institution:
University of Oxford
Division:
MPLS Division
Department:
Engineering Science
More from this funder
Grant:
Seed Award 109908/Z/15/Z to MMK
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Grant:
Young Investigator Award (RGY0073/2015) to MMK
Publisher:
Optical Society Publisher's website
Journal:
Biomedical Optics Express Journal website
Volume:
10
Issue:
1
Pages:
267-267
Publication date:
2018-12-19
Acceptance date:
2018-11-12
DOI:
ISSN:
2156-7085
Pubs id:
pubs:953459
URN:
uri:7e66f6e8-9abc-4764-a2e6-2f8117b95bc0
UUID:
uuid:7e66f6e8-9abc-4764-a2e6-2f8117b95bc0
Local pid:
pubs:953459
Language:
English

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