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Rescue of influenza A virus from recombinant DNA.

Abstract:
We have rescued influenza A virus by transfection of 12 plasmids into Vero cells. The eight individual negative-sense genomic viral RNAs were transcribed from plasmids containing human RNA polymerase I promoter and hepatitis delta virus ribozyme sequences. The three influenza virus polymerase proteins and the nucleoprotein were expressed from protein expression plasmids. This plasmid-based reverse genetics technique facilitates the generation of recombinant influenza viruses containing specific mutations in their genes.
Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
Devenish, L More by this author
Engelhardt, OG More by this author
Brownlee, GG More by this author
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Journal:
Journal of virology
Volume:
73
Issue:
11
Pages:
9679-9682
Publication date:
1999-11-05
EISSN:
1098-5514
ISSN:
0022-538X
URN:
uuid:7db18e72-119f-48cb-a1db-eeaf4328b646
Source identifiers:
20069
Local pid:
pubs:20069

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