Time-resolved fluorescence imaging for specific and quantitative immunodetection of human kallikrein 2 and prostate-specific antigen in prostatic tissue sections.

Journal article

OBJECTIVES: To design protocols for specific and quantitative immunohistochemical detection of human kallikrein 2 (hK2) using lanthanide chelate-labeled monoclonal antibodies (Mabs) and time-resolved fluorescence imaging. METHODS: Anti-prostate-specific antigen (PSA) Mabs were tested in microtiterplate assays for their ability to prevent PSA from cross-reacting with the anti-hK2 Mab 6H10. Europium-labeled 6H10 and terbium-labeled anti-PSA Mab 2E9, selected as the best blocker antibody, were u...

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Authors: Siivola, P; Pettersson, K; Piironen, T; Lövgren, T; Lilja, H

• Journal: Urology
• Volume: 56
• Issue: 4
• Pages: 682-688
• Publication date: 2000-10-01
• DOI: 10.1016/s0090-4295(00)00671-3
• EISSN: 1527-9995
• ISSN: 0090-4295
• Language: English
• Keywords: Humans; Antibodies, Monoclonal; Fluorescence; Tissue Kallikreins; Immunohistochemistry; Prostatic Neoplasms; Male; Tumor Markers, Biological; Prostate; Prostate-Specific Antigen