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Journal article

Probing the proteasome cavity in three steps: bio-orthogonal photo-reactive suicide substrates.

Abstract:
Tri-functional activity-based protein probes that encompass an electrophilic trap, a photo-reactive group and a bio-orthogonal ligation handle are described. With these, and in a three-step chemical proteomics approach, proteasomal catalytic sites are covalently and irreversibly modified, followed by photocrosslinking of these to flanking subunits and Staudinger-Bertozzi ligation for visualization and identification of the resulting conjugates.
Publication status:
Published

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Publisher copy:
10.1039/c0cc03322g

Authors


Journal:
Chemical Communications
Volume:
46
Issue:
47
Pages:
9052-9054
Publication date:
2010-12-01
DOI:
EISSN:
1364-548X
ISSN:
1359-7345
Source identifiers:
103789
Language:
English
Keywords:
Pubs id:
pubs:103789
UUID:
uuid:7d1f4fe0-e35c-4465-8022-dea2952c932a
Local pid:
pubs:103789
Deposit date:
2012-12-19

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