- Abstract:
- Tri-functional activity-based protein probes that encompass an electrophilic trap, a photo-reactive group and a bio-orthogonal ligation handle are described. With these, and in a three-step chemical proteomics approach, proteasomal catalytic sites are covalently and irreversibly modified, followed by photocrosslinking of these to flanking subunits and Staudinger-Bertozzi ligation for visualization and identification of the resulting conjugates.
- Publication status:
- Published
- Publisher copy:
- 10.1039/c0cc03322g
-
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- Journal:
- Chemical communications (Cambridge, England)
- Volume:
- 46
- Issue:
- 47
- Pages:
- 9052-9054
- Publication date:
- 2010-12-05
- DOI:
- EISSN:
-
1364-548X
- ISSN:
-
1359-7345
- URN:
-
uuid:7d1f4fe0-e35c-4465-8022-dea2952c932a
- Source identifiers:
-
103789
- Local pid:
- pubs:103789
- Language:
- English
- Keywords:
- Copyright date:
- 2010
Journal article
Probing the proteasome cavity in three steps: bio-orthogonal photo-reactive suicide substrates.
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