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Quantitative NMR analysis of Erk activity and inhibition by U0126 in a panel of patient-derived colorectal cancer cell lines.

Abstract:

We comparatively analyzed the basal activity of extra-cellular signal-regulated kinase (Erk1/2) in lysates of 10 human colorectal cancer cell lines by semi-quantitative Western blotting and time-resolved NMR spectroscopy. Both methods revealed heterogeneous levels of endogenous Erk1/2 activities in a highly consistent manner. Upon treatment with U0126, an inhibitor of mitogen-activated protein kinase kinase (MEK) acting upstream of Erk1/2, Western-blotting and NMR congruently reported specifi...

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Publication status:
Published

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Journal:
Biochimica et biophysica acta
Volume:
1834
Issue:
7
Pages:
1396-1401
Publication date:
2013-07-01
DOI:
EISSN:
1878-1454
ISSN:
0006-3002
Source identifiers:
388335

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