- Abstract:
-
We comparatively analyzed the basal activity of extra-cellular signal-regulated kinase (Erk1/2) in lysates of 10 human colorectal cancer cell lines by semi-quantitative Western blotting and time-resolved NMR spectroscopy. Both methods revealed heterogeneous levels of endogenous Erk1/2 activities in a highly consistent manner. Upon treatment with U0126, an inhibitor of mitogen-activated protein kinase kinase (MEK) acting upstream of Erk1/2, Western-blotting and NMR congruently reported specifi...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1016/j.bbapap.2013.01.023
-
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- Journal:
- Biochimica et biophysica acta
- Volume:
- 1834
- Issue:
- 7
- Pages:
- 1396-1401
- Publication date:
- 2013-07-05
- DOI:
- EISSN:
-
1878-1454
- ISSN:
-
0006-3002
- URN:
-
uuid:7cffe599-1a95-461c-adab-338df895fbd5
- Source identifiers:
-
388335
- Local pid:
- pubs:388335
- Language:
- English
- Keywords:
- Copyright date:
- 2013
Journal article
Quantitative NMR analysis of Erk activity and inhibition by U0126 in a panel of patient-derived colorectal cancer cell lines.
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