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Bio-imaging of nitric oxide-producing neurones in slices of rat brain using 4,5-diaminofluorescein.

Abstract:

4,5-Diaminofluorescein (DAF-2) was used to identify individual nitric oxide (NO)-producing neurones in brain slices in vitro. Coronal slices of midbrain or hippocampus, 300 microm thick from young adult rats, were incubated for 30 min in 1 microM DAF-2 diacetate (DAF-2 DA) and maintained in ACSF at 33 degrees C. Illumination at 450-490 nm revealed punctate fluorescence in neurones in the lateral tegmental nucleus, dorsal raphe nucleus, dorsolateral periaqueductal grey matter, deep collicular ...

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Publication status:
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Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Neuroscience
Lovick, TA More by this author
Journal:
Journal of neuroscience methods
Volume:
92
Issue:
1-2
Pages:
101-110
Publication date:
1999-10-05
DOI:
EISSN:
1872-678X
ISSN:
0165-0270
URN:
uuid:7b73e63f-7786-48f5-9d15-4262d401eee5
Source identifiers:
102622
Local pid:
pubs:102622

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