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Effect of slow freezing versus vitrification on the recovery of mouse embryonic stem cells

Abstract:

The purpose of this study was to cryopreserve mouse embryonic stem (ES) cells R1 line and determine cell viability, morphology, the number of colonies, and the Alkaline Phosphatase (AP) activity. In addition, the expression of transcription factors such as the stage-specific antigen (SSEA-1) and Octamer-4 (Oct-4) were evaluated before and after cryopreservation. The effects of two methods of cryopreservation, slow freezing and vitrification, were studied. The ES cells were cryopreserved eithe...

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Publication status:
Published

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Publisher copy:
10.1089/cpt.2006.9990

Authors


Miszta-Lane, H More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Primary Care Health Sciences
Mirbolooki, M More by this author
Lakey, JRT More by this author
Journal:
CELL PRESERVATION TECHNOLOGY
Volume:
5
Issue:
1
Pages:
16-24
Publication date:
2007
DOI:
EISSN:
1557-8119
ISSN:
1538-344X
URN:
uuid:7ab88111-f67b-4f8f-8b52-0ed7daf44c51
Source identifiers:
244274
Local pid:
pubs:244274
Language:
English

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