L-delta-(alpha-Aminoadipoyl)-L-cysteinyl-D-valine synthetase: thioesterification of valine is not obligatory for peptide bond formation.

Journal article

L-delta-(alpha-Aminoadipoyl)-L-cysteinyl-D-valine (ACV) synthetase is probably the simplest known peptide synthetase in terms of the number of reactions catalyzed. In the "thiol-template" proposal for nonribosomal peptide synthesis, a key step is transfer of aminoacyl groups derived from the substrates to enzyme-bound thiols prior to peptide bond formation. No incorporation of 18O was seen in AMP isolated from the reaction mixture when di[18O]valine was incubated with relatively large amounts...

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Authors: Shiau, C; Byford, M; Aplin, RT; Baldwin, J; Schofield, C

• Publication status: Published
• Journal: Biochemistry
• Volume: 36
• Issue: 29
• Pages: 8798-8806
• Publication date: 1997-07-01
• DOI: 10.1021/bi962932e
• EISSN: 1520-4995
• ISSN: 0006-2960
• Language: English
• Keywords: 2-Aminoadipic Acid; Serine; Binding Sites; Valine; Models, Chemical; Acremonium; Protein Binding; Adenosine Triphosphate; Peptide Synthases