\textbf{LB Medium}

Bacto-tryptone 10g/l

Yeast extract 5g/l 

NaCl 5g/l

pH 7.0 
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\textbf{Succinate Medium}

1M Phosphate buffer 20ml

Concentrated base 20ml

Ammonium sulphate 0.5g

Growth factors 2ml

Sodium succinate 2g

NaCl 0.5g

Casamino acids 1g

Made up to 1l, pH 7.2
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\textbf{M22 Medium}

As for succinate medium but omitting sodium succinate and casamino acids.
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\textbf{Concentrated Base}

Nitriloacetic acid (disodium salt) 5.94g

Metals 44 solution 25ml

MgSO$_4$.7H$_2$O 14.5g

CaCl$_2$.6H$_2$O 2.5g

FeSO$_4$.7H$_2$O 50mg

Ammonium molybdate (4H$_2$0) 4.6mg

Made up to 1l, pH 6.8. Store at 4 \degc{}.
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\textbf{Metals 44 Solution}

EDTA 2.5g

ZnSO$_4$.7H$_2$O 11g

FeSO$_4$.7H$_2$O 5g

MnSO$_4$.4H$_2$O 2g

CuSO$_4$.5H$_2$O 0.39g

CoCl$_2$.6H$_2$O 0.2g

Boric acid 0.12g

Sulphuric acid 1.5ml

Made up to 1l and filter sterilised. Store at 4 \degc{}.
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\textbf{Growth Factors}

NaHCO$_3$ 50mg

Biotin 2mg

Niacin 100mg

Thiamine 50mg

Made up to 100ml and filter sterilised. Store at 4 \degc{}.
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\textbf{1M Phosphate Buffer}

Buffer A 136.8g K$_2$HPO$_4$.3H$_2$O in 600ml water

Buffer B 68g KH$_2$PO$_4$ in 500ml water

Mix 61.5ml Buffer A with 38.5ml Buffer B, adjust to pH 7.0 and store at 4 \degc{}.
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\textbf{HEPES}

4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid 2.38g in 1l water, pH 7.2 (NaOH)
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\textbf{5X SDS-PAGE Loading Dye}

Glycerol 25ml

0.5M Tris pH 6.8 22.5ml

SDS 2.5g

$\beta$-mercaptoethanol 2.5g
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\textbf{Phosphate Buffered Saline (PBS) Buffer}

NaCl 8g

KCl 0.2g

Na$_2$HPO$_4$.12H$_2$O 1.44g 

KH$_2$PO$_4$ 0.24g

Made up to 1l, pH 7.4