T-cell triggering thresholds are modulated by the number of antigen within individual T-cell receptor clusters.

Journal article

T cells react to extremely small numbers of activating agonist peptides. Spatial organization of T-cell receptors (TCR) and their peptide-major histocompatibility complex (pMHC) ligands into microclusters is correlated with T-cell activation. Here we have designed an experimental strategy that enables control over the number of agonist peptides per TCR cluster, without altering the total number engaged by the cell. Supported membranes, partitioned with grids of barriers to lateral mobility, p...

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Authors: Manz, B; Jackson, B; Petit, R; Dustin, M; Groves, J

• Journal: Proceedings of the National Academy of Sciences of the United States of America
• Volume: 108
• Issue: 22
• Pages: 9089-9094
• Publication date: 2011-05-01
• DOI: 10.1073/pnas.1018771108
• EISSN: 1091-6490
• ISSN: 0027-8424
• Language: English
• Keywords: Ligands; Calcium; Lipids; Lipid Bilayers; Calcium Signaling; CD4-Positive T-Lymphocytes; T-Lymphocytes; Mice; Major Histocompatibility Complex; Lymphocyte Activation; Intercellular Adhesion Molecule-1; Biophysics; Animals; Receptors, Antigen, T-Cell; Immune System