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The molecular dissection of TRIM25’s RNA-binding mechanism provides key insights into its antiviral activity

Abstract:

TRIM25 is an RNA-binding ubiquitin E3 ligase with central but poorly understood roles in the innate immune response to RNA viruses. The link between TRIM25’s RNA binding and its role in innate immunity has not been established. Thus, we utilized a multitude of biophysical techniques to identify key RNA-binding residues of TRIM25 and developed an RNA-binding deficient mutant (TRIM25-m9). Using iCLIP2 in virus-infected and uninfected cells, we identified TRIM25’s RNA sequence and structure spec...

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Publication status:
Published
Peer review status:
Peer reviewed

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Role:
Author
ORCID:
0000-0002-1437-5773
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Role:
Author
ORCID:
0000-0001-5025-3821
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Institution:
University of Oxford
Role:
Author
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Role:
Author
ORCID:
0000-0001-9413-4920
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Role:
Author
ORCID:
0000-0003-4082-1893
Publisher:
Nature Research
Journal:
Nature Communications More from this journal
Volume:
15
Issue:
1
Article number:
8485
Publication date:
2024-10-01
Acceptance date:
2024-09-23
DOI:
EISSN:
2041-1723
Language:
English
Source identifiers:
2299947
Deposit date:
2024-10-01
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