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Surface labeling of key residues during assembly of the transmembrane pore formed by staphylococcal alpha-hemolysin.

Abstract:

Structural changes in staphylococcal alpha-hemolysin (alpha HL) that occur during oligomerization and pore formation on membranes have been examined by using a simple gel-shift assay to determine the rate of modification of key single-cysteine mutants with the hydrophilic sulfhydryl reagent, 4-acetamido-4'-((iodoacetyl)amino)stilbene-2,2'-disulfonate (IASD). The central glycine-rich loop of alpha HL lines the lumen of the transmembrane channel. A residue in the loop remains accessible to IASD...

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Publication status:
Published

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Authors


Krishnasastry, M More by this author
Journal:
FEBS letters
Volume:
356
Issue:
1
Pages:
66-71
Publication date:
1994-12-05
DOI:
EISSN:
1873-3468
ISSN:
0014-5793
URN:
uuid:7986eac6-9da1-4557-89a7-e505ed3f67e8
Source identifiers:
52279
Local pid:
pubs:52279

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