The effects of serum, lithium, ethacrynic acid, and a low external concentration of potassium on specific [3H]-ouabain binding to human lymphocytes after incubation for 3 days.

Journal article

We have quantified specific [3H]-ouabain binding sites in normal human lymphocytes, and have measured the changes in the numbers of those sites which occur in response to various stimuli. We have confirmed previous findings that incubation for 72 h in the presence of fetal calf serum causes an increase in [3H]-ouabain binding, and that this does not occur if the cells are incubated in fetal calf serum which has first been dialysed. During incubation of the lymphocytes for 3 days in the presen...

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Authors: Rapeport, W; Aronson, J; Grahame-Smith, D; Harper, C

• Publication status: Published
• Journal: British journal of clinical pharmacology
• Volume: 22
• Issue: 3
• Pages: 275-279
• Publication date: 1986-09-01
• DOI: 10.1111/j.1365-2125.1986.tb02887.x
• EISSN: 1365-2125
• ISSN: 0306-5251
• Language: English
• Keywords: Lithium; Adult; Acridines; Binding Sites; Male; Ouabain; Humans; Aged; Adolescent; Middle Aged; Time Factors; Potassium; Lymphocytes; Female; Ethacridine