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Physical evidence for the induced release of the Bacillus subtilis transcription factor, sigma(F), from its inhibitory complex.

Abstract:

The release of the transcription factor sigma(F) from its inhibitory complex with SpoIIAB is a key regulatory step in the control of sporulation in Bacillus subtilis as it initiates a pattern of differential gene expression in the mother cell and prespore compartments. The sigma(F).SpoIIAB complex is dissociated by the unphosphorylated form of the protein SpoIIAA, the alternative binding partner of SpoIIAB. Here, we employ fluorescence spectroscopy to examine the mechanism by which SpoIIAA ac...

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Publisher copy:
10.1016/j.jmb.2004.04.061

Authors


Clarkson, J More by this author
Campbell, ID More by this author
Yudkin, MD More by this author
Journal:
Journal of molecular biology
Volume:
340
Issue:
2
Pages:
203-209
Publication date:
2004-07-05
DOI:
EISSN:
1089-8638
ISSN:
0022-2836
URN:
uuid:790be4fb-2458-4d44-bb81-3717f0e4db57
Source identifiers:
246469
Local pid:
pubs:246469

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