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Metabolic network fluxes in heterotrophic Arabidopsis cells: stability of the flux distribution under different oxygenation conditions.

Abstract:

Steady-state labeling experiments with [1-(13)C]Glc were used to measure multiple metabolic fluxes through the pathways of central metabolism in a heterotrophic cell suspension culture of Arabidopsis (Arabidopsis thaliana). The protocol was based on in silico modeling to establish the optimal labeled precursor, validation of the isotopic and metabolic steady state, extensive nuclear magnetic resonance analysis of the redistribution of label into soluble metabolites, starch, and protein, and a...

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Publication status:
Published

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Publisher copy:
10.1104/pp.108.125195

Authors


Williams, TC More by this author
Masakapalli, SK More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MPLS, Plant Sciences
More by this author
Institution:
University of Oxford
Department:
Oxford, MPLS, Plant Sciences
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Journal:
Plant physiology
Volume:
148
Issue:
2
Pages:
704-718
Publication date:
2008-10-05
DOI:
EISSN:
1532-2548
ISSN:
0032-0889
URN:
uuid:78ab9b38-38e1-4252-a91e-286cb46e6e11
Source identifiers:
34335
Local pid:
pubs:34335

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