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Fluorescent imaging of nitric oxide production in neuronal varicosities associated with intraparenchymal arterioles in rat hippocampal slices.

Abstract:

The fluorescent indicator 4,5-diaminofluorescein (DAF-2) has been used to investigate the production of nitric oxide in the vicinity of intraparenchymal cerebral blood vessels. Slices of rat hippocampus 300-350 microm thick, were loaded with 5 microM DAF-2 diacetate. On exposure to light of 450-490 nm wavelength, point sources of fluorescence, 1.8+/-0.2 microm in diameter (mean+/-SEM), were observed in close apposition to the outer surface of the vascular smooth muscle wall of 10/15 arteriole...

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Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Neuroscience
Role:
Author
Journal:
Neuroscience letters
Volume:
294
Issue:
1
Pages:
9-12
Publication date:
2000-11-05
DOI:
EISSN:
1872-7972
ISSN:
0304-3940
URN:
uuid:776f8412-3b9a-4e4a-9ac6-d2951606a3d2
Source identifiers:
102623
Local pid:
pubs:102623

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