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Comprehensive human adipose tissue mRNA and microRNA endogenous control selection for quantitative real-time-PCR normalization.

Abstract:

The accurate quantification of cellular and tissue mRNA and microRNA content is reliant upon the selection of stable endogenous control transcripts for normalizing quantitative real-time-PCR (qRT-PCR) data. Using the combination of unbiased and informed approaches and a wide range of human adipose tissues and cells, we sought to identify invariant control transcripts for mRNA and microRNA. A total of 26 mRNA transcript candidates were selected from the literature. MicroRNA candidates were sel...

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Publication status:
Published

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Publisher copy:
10.1038/oby.2010.257

Authors


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Institution:
University of Oxford
Department:
Oxford, MSD, RDM, OCDEM
Role:
Author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, RDM, OCDEM, Biomedical Research Centre
Role:
Author
Journal:
Obesity (Silver Spring, Md.)
Volume:
19
Issue:
4
Pages:
888-892
Publication date:
2011-04-05
DOI:
EISSN:
1930-739X
ISSN:
1930-7381
URN:
uuid:76b8abf3-e951-47b9-abfd-c3c6dda09ec7
Source identifiers:
90772
Local pid:
pubs:90772

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