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Rates and Stoichiometries of Metal Ion Probes of Cysteine Residues within Ion Channels

Abstract:

Metal ion probes are used to assess the accessibility of cysteine side chains in polypeptides lining the conductive pathways of ion channels and thereby determine the conformations of channel states. Despite the widespread use of this approach, the chemistry of metal ion-thiol interactions has not been fully elucidated. Here, we investigate the modification of cysteine residues within a protein pore by the commonly used Ag+ and Cd2+ probes at the single-molecule level, and provide rates and s...

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Publication status:
Published
Peer review status:
Peer reviewed

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Files:
Publisher copy:
10.1016/j.bpj.2013.04.046

Authors


Publisher:
Elsevier Publisher's website
Journal:
Biophysical Journal Journal website
Volume:
105
Issue:
2
Pages:
356-364
Publication date:
2013-07-16
Acceptance date:
2013-04-25
DOI:
EISSN:
1542-0086
ISSN:
0006-3495
Pmid:
23870257
Source identifiers:
414517

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