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Journal article

A device for extraction, manipulation and stretching of DNA from single human chromosomes.

Abstract:

We describe the structure and operation of a micro/nanofluidic device in which individual metaphase chromosomes can be isolated and processed without being displaced during exchange of reagents. The change in chromosome morphology as a result of introducing protease into the device was observed by time-lapse imaging; pressure-driven flow was then used to shunt the chromosomal DNA package into a nanoslit. A long linear DNA strand (>1.3 Mbp) was seen to stretch out from the DNA package and a...

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Publication status:
Published

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Publisher copy:
10.1039/c0lc00603c

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Journal:
Lab on a chip
Volume:
11
Issue:
8
Pages:
1431-1433
Publication date:
2011-04-05
DOI:
EISSN:
1473-0189
ISSN:
1473-0197
URN:
uuid:76110b0e-141e-43e8-95ba-8d7300a3adfa
Source identifiers:
120176
Local pid:
pubs:120176

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