Model generation of viral channel forming 2B protein bundles from polio and coxsackie viruses.

Journal article

2B is a 99 amino acid membrane protein encoded by enteroviruses such as polio and coxsackie viruses with two transmembrane domains. The protein is found to make membranes of infected cells permeable. Using a computational approach which positions the models and assesses stability by molecular dynamics (MD) simulations a putative tetrameric bundle model of 2B is generated. The bundles show a pore lining motif of three lysines followed by a serine. The bundle is discussed in terms of different possible orientations of the helices in the membrane and the consequences this has on the in vivo activity of 2B.

Authors: Patargias, G; Barke, T; Watts, A; Fischer, W

• Publication status: Published
• Journal: Molecular membrane biology
• Volume: 26
• Issue: 5
• Pages: 309-320
• Publication date: 2009-08-01
• DOI: 10.1080/09687680903164101
• EISSN: 1464-5203
• ISSN: 0968-7688
• Language: English
• Keywords: Enterovirus; Models, Molecular; Ion Channels; Amino Acid Sequence; Computer Simulation; Poliovirus; Protein Structure, Tertiary; Computational Biology; Lysine; Protein Multimerization; Molecular Sequence Data; Protein Structure, Secondary; Viral Proteins; Viral Nonstructural Proteins; Static Electricity