A sequence pattern for peptides presented to cytotoxic T lymphocytes by HLA B8 revealed by analysis of epitopes and eluted peptides.

Journal article

HLA B8-restricted cytotoxic T lymphocytes (CTL) specific for influenza A virus were generated and shown to recognize the nucleoprotein (NP). The dominant epitope was mapped using recombinant vaccinia viruses that expressed fragments of the NP and then synthetic peptides based on the NP amino acid sequence. The peptide 380-393 was first identified and further refined; it was shown that the glutamic acid at position 380 was essential for recognition by CTL and that the nonamer 380-388 was the o...

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Authors: Sutton, J; Rowland-Jones, S; Rosenberg, W; Nixon, D; Gotch, F

• Journal: European journal of immunology
• Volume: 23
• Issue: 2
• Pages: 447-453
• Publication date: 1993-02-01
• DOI: 10.1002/eji.1830230222
• EISSN: 1521-4141
• ISSN: 0014-2980
• Language: English
• Keywords: Protein Conformation; RNA-Binding Proteins; Structure-Activity Relationship; Epitopes; HIV Infections; HLA-B8 Antigen; Humans; Peptides; HIV-1; T-Lymphocytes, Cytotoxic; Molecular Sequence Data; Amino Acid Sequence; Viral Vaccines; Antigen-Presenting Cells; Nucleoproteins; Influenza A virus; Viral Core Proteins; Gene Expression